Multi-antisera applicator for use in solid-support immunoelectrophoresis on cellulose acetate.

نویسندگان

  • S T Nerenberg
  • C Ganger
  • L DeMarco
چکیده

We describe for the first time a multi-antisera applicator to be used on cellulose acetate membranes with immunoelectrophoresis on a solid support. The new procedure overcomes previous shortcomings of immunoelectrophoresis on cellulose acetate membranes and permits rapid analyses of 11 samples on single “standard”-size membranes with a substantialsaving in antisera, celluloseacetate membranes, and time.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Joachim kohn (1912-1987) and the origin of cellulose acetate electrophoresis.

The year 2006 marks the 50th anniversary of the discovery of cellulose acetate (CA) electrophoresis by Joachim Kohn, a pathologist at Queen Mary's Hospital in Roehampton, London. During a career in pathology that began in 1950 and spanned 37 years, Kohn published more than 50 papers in clinical laboratory medicine. He was the first to report the use of CA microbiology filters as solid supports ...

متن کامل

Rapid large-scale screening of blood proteins by use of cellulose acetate electrophoresis on a solid support.

We describe a new principle of cellulose acetate electrophoresis (solid-support electrophoresis), in which cellulose acetate membranes are given a solid support, instead of being suspended as heretofore. The new technique is particularly well suited for screening procedures, because many samples can be processed economically on a single run. Also described for the first time, in conjunction wit...

متن کامل

Detection and identification of monoclonal components: immunoelectrophoresis on agarose gel and immunofixation on cellulose acetate compared.

Immunofixation on cellulose acetate has been compared with immunoelectrophoresis on agarose gel. We studied 90 sera containing monoclonal components and 10 containing polyclonal immunoglobulins according to agarose gel screening electrophoresis. Results by the two methods agreed about 80% of the time for monoclonal components in concentrations exceeding 1-2 g/L. Immunofixation appeared more sen...

متن کامل

Synthesis Magnesium Hydroxide Nanoparticles and Cellulose Acetate- Mg(OH)2-MWCNT Nanocomposite

Mg(OH)2 nanoparticles were synthesized by a rapid microwave reaction. The effect of sodium dodecyl sulfonate (SDS as anionic surfactant) and cetyl tri-methyl ammonium bromide (CTAB as cationic surfactant) on the morphology of magnesium hydroxide nanostructures was investigated. Multi wall carbon nano tubes was organo-modified for better dispersion in cellulose acetate matrix. The influence of M...

متن کامل

Benign paraproteinaemias.

A paraprotein can be defined in our present state of knowledge as a complete or incomplete immunoglobulin molecule, possibly abnormal, produced in excess by a single clone of immunocytes (Osserman and Fahey, 1968), and characterized on electrophoresis by a discrete band or peak. The discovery of a paraprotein in the plasma should not be regarded as synonymous with a malignant proliferative dise...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Clinical chemistry

دوره 18 9  شماره 

صفحات  -

تاریخ انتشار 1972